PARP (Cleaved) Antibody

Synonimy: PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1, Poly [ADP-ribose] polymerase 1; PARP-1; ADPRT 1; Poly[ADP-ribose] synthase 1
Typ przeciwciała: Polyclonal
Pochodzenie: Rabbit
Izotyp: Rabbit IgG
Klon:
Reaktywność: Rat
Immunogen: Synthetic peptide corresponding to the C-terminus surrounding Asp214 of rat PARP
NCBI: P27008
Gene ID: 3123251
Stężenie: 0.2 mg/ml
100 µg (0.2 mg/ml) affinity purified, polyclonal rabbit anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
Przeznaczenie: Western blot, Immunocytochemistry
Western blot analysis (1µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.
For Research Use Only! Not to be used in humans.
Informacje: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Temp. przechowywania: -20 °C
Czas przydatności: 12 months