Caspase-9 (Active) Antibody

Synonimy: CASP-9,EC 3.4.22.62,ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3, Apaf-3
Typ przeciwciała: Polyclonal
Pochodzenie: Rabbit
Izotyp: Rabbit IgG
Klon:
Reaktywność: Rat
Immunogen: Synthetic peptide corresponding to amino-terminal residues adjacent to Asp353 of rat caspase-9
NCBI: AAK26235.1
Gene ID: 18450366
Stężenie: 0.2 mg/ml
100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Przeznaczenie: Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml), Immunocytochemistry (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes the cleaved caspase-9 (37 kDa/17 kDa). It does not recognize full-length caspase-9 or any other caspases.
For Research Use Only! Not to be used in humans.
Informacje: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Temp. przechowywania: -20 °C
Czas przydatności: 12 months