FtsH2 | FtsH2 positive control/quantitation standard

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Aplikacje: western blot (WB)
Zalecane rozcieńczenie: Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a FtsH2 signal in this range.Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems.
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Przechowywanie: store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Uwagi: The FtsH2 protein standard can be used in combination with anti-FtsH2 antibodies to quantitate FtsH2 from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in the FtsH protein.Quantitative western blot: detailed method description, video tutorial
Powiązane produkty: AS07 205 | anti-FtsH4 | plant fts metallo-protease H4AS07 251 | anti-FtsH10 | plant fts metallo-protease H10
Dodatkowe informacje: Concentration: after adding 225 µl of sterile milliQ water final concentration of the standard is 0.1 pmoles/µlThis standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.

FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Literatura: to be added when available, antibody released in October 2011.