CO4c | Complement component C4c antibody

Immunogen: full lenth native protein P0C0L4 purified from human plasma
Źródło: hen
Klonalność: polyclonal
Reaktywność: human
Przewidywana reaktywność: human
Czystość: total IgY fraction at 25.9 mg/ml in 0.9% NaCl, 0.1% sodium azide
Postać: liquid
Aplikacje: Western blot (WB)
Zalecane rozcieńczenie: use at an assay dependent dilution (WB)
Nr UniProt:
Nr TAIR:
Przechowywanie: store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Uwagi: the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation
Powiązane produkty: IMS02-032-303 | anti-Complement component C4IMS06-032-304 | anti-Complement component C4, biotinylatedIMS01-066-309 | anti-Complement factor H
Dodatkowe informacje: Only one precipitin arcs in immunodiffusion against normal human serum or human C4. Reacts with α and β chain in electroblotting.

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Literatura: Bidula et al. (2013). The role of ficolin-A and lectin complement pathway in the innate defence against pathogenic Aspergillus species. Infect. Immun. March 11.