EZCut™ TEV Protease, Recombinant

Alternate Name/Synonyms: Nuclear inclusion protein A, NIa protein

Gene Symbol: N/A

Accession #: N/A

Gene ID: N/A

Source: E. coli

Appearance: Liquid

Physical Form Description: 1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0

Molecular Weight: 28.6 kDa (2038–2279 aa + C-terminal poly-his tag).

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: N/A

Biological Activity: The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.

Reconstitution Instructions: N/A

Storage Temp.: -80°C

Shipping: dry ice

Background Information: BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

Amino Acid Sequence: 2038–2279 aa

Handling: Centrifuge the vial prior to opening

USAGE: For Research Use Only! Not For Use in Humans.