JMJD2A Antibody

Synonimy: Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A
Typ przeciwciała: Polyclonal
Pochodzenie: Rabbit
Izotyp: Rabbit IgG
Klon:
Reaktywność: Human JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10% cross reactivity with recombinant JMJD2D, however bands were detected at 60 kDa from cell samples.
Immunogen: Human recombinant JMJD2A aa 1-350
NCBI: O75164
Gene ID: 9682
Stężenie: N/A
50 µg of antibody in 250 μl TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide.
Przeznaczenie: Western blot
Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins.
For Research Use Only! Not to be used in humans.
Informacje: Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.
Temp. przechowywania: -20 °C
Czas przydatności: 12 months