2 | Photosynthesis Tool Kit - quantitation

Immunogen: Źródło: serum or affinity purified antibodies Lyophilized, please add sterile water for reconstitution of antibodies and protein standards following information on each tube.The secondary antibody is in liquid format
Klonalność:
Reaktywność: higher plants, algae, cyanobacteria
Przewidywana reaktywność:
Czystość:
Postać:
Aplikacje: western blot (WB)
Zalecane rozcieńczenie:
Nr UniProt: O03042 , P05698 , P0C510 , P00877, P62090 , P69416 , P0C360 , Q00914 , Q31QV2, P83755 , P0C434 , Q14FH6, Q6YXN7 , P07753 , P14660
Nr TAIR: ATCG00490, ATCG01060,ATCG00020,
Przechowywanie: store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Uwagi: Product information - Primary antibodies:Product nuumber:Product name:Reconstitution: Recommended dilution:AS03 037Rabbit Anti-RbcL Global antibodyFor reconstitution see lable on respective tube.1:5000 for ECL (2 µl for 10ml)AS10 939Rabbit Anti-PsaC Global antibodyFor reconstitution see lable on respective tube.1:5000 for ECL (2 µl for 10ml)AS05 084Rabbit Anti-PsbAGlobal antibodyFor reconstitution see lable on respective tube.1:5000 for ECL (2 µl for 10ml)* All primary antibodies in this kit are raised in rabbits.Product information - Protein standards:Product number:Product name:Concentration:Size:Western Blot – Positive Control:AS01 016SPsbA *0.25 pmol/ μl41.5 kDa#To generate a standard curve, 3 loads are suggested (0.5, 1.0 and 3.0 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.A 3 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS01 017SRbcL *0.15 pmol/ μl52.7 kDaTo generate a standard curve, 3 loads are suggested (4, 10 and 20 ul). For most applications a sample load of 0.1 ug of chlorophyll will give a RbcL signal in this range.A 10 ul load is optimal for most chemiluminescent detection systems to use as a positive control.AS04 042SPsaC *0.15 pmol/μl11.5 kDa#To generate a standard curve, 3 loads are suggested (5, 10 and 15ml). For most applications a sample load of 0.2mg of chlorophyll will give a PsaC signal in this range.A 5 ul load is optimal for most chemiluminescent detection systems as a positive control.*These proteins are larger than a respective native protein due to the addition of His-tag* Reconstitution of standards: Please add 90 µl of sterile water for reconstitution.Product information - Secondary antibody:AS09 602- Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 µlEducational information about Quantitative western blot can be found here: detailed method description, video tutorial
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This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique. The kit contains following global antibodies, protein standards and secondary anribody: Rabbit anti-RbcL (Form I and Form II). Product number: AS03 037 Rabbit anti-PsaC. Product number: AS10 939Rabbit anti-PsbA Product number: AS05 084Rubisco protein standard. Product number: AS01 017S PsaC protein standard. Product number: AS04 042S PsbA protein standard. Product number: AS01 016S Goat anti-Rabbit IgG (H&L), HRP conj. (50 µl), Product number: AS09 602
Literatura: MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202. Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951. Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)