GlnA | glutamine synthetase antibody

Immunogen: KLH-conjugated synthetic peptide derived from available bacterial GlnA sequences with perfect conservation in alpha, beta, gamma Proteobacteria, Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weak conservation with Trichodesmium thiebautii) including Synechocystis PCC 6803 Q59981
Źródło: hen
Klonalność: polyclonal
Reaktywność: Synechococcus sp. 7942, Synechocystis sp. 6803
Przewidywana reaktywność: alpha, beta, gamma proteobacteria, enterobacteria, thermotogales, euryarchaeotes, crenarchaeotes, Arthropsira sp. PCC 8005
Czystość: total IgY in PBS pH 8.0+ 0.02% sodium azide, conc.16 µg/µl
Postać: liquid
Aplikacje: western blot (WB)
Zalecane rozcieńczenie: 1:5000 with standard ECL (WB)
Nr UniProt: P77961 , Q59981
Nr TAIR:
Przechowywanie: store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Uwagi: Peptide target used to elicit this antibody has a weak, sporadic conservation with Glutamine Synthetase to III, antibody not expected to detect this enzyme. Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GLNRS), but this antibody is not expected to detect this enzyme.
Powiązane produkty: AS01 018S | GlnA protein standard for a quantitative western blot or as a positive controlRecommended secondary antibodycollection of antibodies to nitrogen metabolism
Dodatkowe informacje:

Glutamine synthetase (EC=6.3.1.2) is the key enzyme in the incorporation of mineral nitrogen into glutamine. Activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.
Literatura: Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biol. 154:413-422.Burns et al. (2006). Inorganic carbon repletion constrains steady-state light acclimation in the cyanobacterium Synechococcus elongatus. J. Phycol. 42:610-621.