Przeznaczenie
Ilościowe określanie aktywności enzymatycznej dehydrogenazy sorbitolu.
• For quantitative determination of sorbitol dehydrogenase enzyme activity.
Zalety
• Fast and sensitive. Linear detection range (20 μL sample): 0.1 to 125 U/L for 12 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Metoda
• OD565nm
Źródło prób
Próbki pochodzenia biologicznego, np. osocze krwi, surowica krwi, mocz, tkanki i kultury tkankowe.
• Biological samples e.g. plasma, serum, urine, tissue and culture media.
Gatunki
Wszystkie
• All
Czas wykonania testu
• 15 min
Ilość testów
• 100 tests
Limit detekcji
• 0.1 U/L
Czas przechowywania
6 miesięcy
• 6 months
Informacje dodatkowe
Dehydrogenaza sorbitolu (SDH)
• SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. BioAssay Systems’ non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
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101bioGentaur Sp. z o. o.
Ul. Grunwaldzka 88A/2Centrala (Belgia)
Voortstraat 49, 1910 Kampenhout BELGIUM